circRNA basic information
circBase ID: -
Name: hsa_circ_WDR78
Synonym: ASCRP000647
Host Gene: WDR78
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; normal tissues
Cell lines:

A549; H2170
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: FBXW8, HIF1a, CD274 (PD-L1), Ago 2
Associated microRNA: miR-1265
Biological function: circ-WDR78 exhibits anti-tumor effects in NSCLC cells, suppressing cell proliferation, colony formation, invasion, and migration, promoting apoptosis, inducing ROS accumulation and autophagy, and helping prevent immune escape through PD-L1 inhibition.
Molecular mechanism: circ-WDR78 functions as a ceRNA that sponges miR-1265 to derepress FBXW8, promoting HIF1a degradation; TRQ-induced exosomal circ-WDR78 inactivates HIF1a signaling, increases ROS and autophagy, and reduces HIF1a-dependent PD-L1 expression.
Biological pathway or process:

HIF-1 signaling (inhibits); autophagy (promotes); apoptosis (promotes); proliferation (inhibits); migration (inhibits); invasion (inhibits); immune regulation (promotes); ceRNA regulation (other); ubiquitination (promotes)
Detected method:
Q
M
Validation methods:

RT-qPCR; Bioinformatics Analysis; Actinomycin D / DRB Stability Assay; RNase R Treatment; Transfection; CCK8; Colony Formation Assay; Transwell Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; RNA Pull-Down; ChIP / ChIP-seq; IF (Immunofluorescence); Flow Cytometry（Non-apoptosis/cycle）; Western Blot
Clinical significance:

-
Description:

circ-WDR78 is a TRQ-induced, exosome-transmitted circRNA with tumor-suppressive activity in NSCLC cells. It sponges miR-1265 to derepress FBXW8, promoting HIF1a degradation, ROS accumulation, autophagy, and reduced PD-L1-mediated immune escape, thereby inhibiting proliferation, invasion, and migration while promoting apoptosis.
Confidence score:

0.7998
Other information
Title:

Tanreqing suppresses the proliferation and migration of non-small cell lung cancer cells by mediating the inactivation of the HIF1alpha signaling pathway via exosomal circ-WDR78.

Journal: Journal of biomolecular structure & dynamics
Published: 2025
PubMed ID: 38247231
Study type:

biological research
Data availability: GSE112214
Code availability: -