| Expression pattern: |
DN |
| Associated gene: |
SRRM4 |
| Associated microRNA: |
- |
| Biological function: |
unknown |
| Molecular mechanism: |
SRRM4-dependent microexon inclusion at the backsplice junction promotes ME-circEIF4G3 biogenesis. |
| Biological pathway or process: |
other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RNA-seq; Bioinformatics Analysis; RNase R Treatment; RT-qPCR; divergent primers PCR; Sanger Sequencing; Clinical Sample Validation; Transfection |
| Clinical significance: |
ME-circEIF4G3 is reduced in matched GBM tumor versus healthy brain tissue in an example patient, and is proposed for further investigation in gliomagenesis. |
| Description: |
ME-circEIF4G3 (a microexon-containing circRNA from EIF4G3) is biogenesis-controlled by the splicing factor SRRM4. It shows reduced abundance in matched GBM tumor versus healthy brain tissue in an example patient, and was experimentally validated by RT-PCR and Sanger sequencing across the backsplice junction. |
| Confidence score: |
0.6926 |