| Expression pattern: |
UP |
| Associated gene: |
HOTTIP, RAB11FIP1 |
| Associated microRNA: |
miR-497-5p |
| Biological function: |
promotes PDAC cell growth, migration, invasion, tumor growth and liver metastasis; promotes EMT |
| Molecular mechanism: |
acts as a sponge for miR-497-5p to promote lncRNA HOTTIP (circRNA-miRNA-lncRNA pathway); binds RAB11FIP1 and blocks its ubiquitination site to stabilize RAB11FIP1 |
| Biological pathway or process: |
EMT (promotes); metastasis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (other); ceRNA regulation (promotes); ubiquitination (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Co-IP |
| Clinical significance: |
PDAC patients with liver metastasis had significantly upregulated circRTN4 expression level in their primary tumors; potential biomarker and therapeutic target for PDAC |
| Description: |
circRTN4 is up-regulated in PDAC tumors and promotes malignant phenotypes (proliferation, migration/invasion, tumor growth and liver metastasis). Mechanistically, it sponges miR-497-5p to increase oncogenic lncRNA HOTTIP expression and also binds RAB11FIP1 to inhibit its ubiquitination and stabilize the protein, thereby promoting EMT marker expression. |
| Confidence score: |
0.8784 |