circRNA basic information
circBase ID: hsa_circ_0000517
Name: hsa_circ_RPPH1
Synonym: circRPPH1
Host Gene: RPPH1
Genomic location(hg19): chr14:20811404-20811492:-
Genomic location(hg38): chr14:20343245-20343333:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small-cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; normal tissues; clinical samples
Cell lines:

BEAS-2B; PC9; H460; A549; H1299
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ERBB4, Ago2
Associated microRNA: miR-326
Biological function: Promotes NSCLC cell proliferation, viability, colony formation, migration, invasion, metastatic/aggressive behavior, and tumor growth; knockdown suppresses these malignant phenotypes.
Molecular mechanism: circRPPH1 functions as a ceRNA by sponging miR-326, thereby regulating ERBB4 expression through the miR-326/ERBB4 signal axis.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EGFR (promotes)
Detected method:
Q
M
Validation methods:

Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis
Clinical significance:

High circRPPH1 expression is associated with TNM stage, lymphatic metastasis, lower overall survival, and may serve as a diagnostic and prognostic biomarker for NSCLC.
Description:

circRPPH1 is up-regulated in NSCLC tissues and cells and is associated with advanced TNM stage, lymphatic metastasis, and poor overall survival. Functionally, circRPPH1 promotes NSCLC cell proliferation, migration, invasion, and xenograft tumor growth. Mechanistically, it acts through a ceRNA mechanism by sponging miR-326 and regulating ERBB4 expression via the miR-326/ERBB4 axis.
Confidence score:

0.8914
Other information
Title:

Silencing of circRPPH1 Inhibits the Progression of Non-Small-Cell Lung Cancer Through miR-326/ERBB4 Signal Axis.

Journal: Biochemical genetics
Published: 2025
PubMed ID: 38776052
Study type:

combined biological and clinical study
Data availability: GSE158695
Code availability: -