| Expression pattern: |
UP |
| Associated gene: |
NUPR1, PI3K, AKT, Ago2 |
| Associated microRNA: |
miR-637 |
| Biological function: |
Promotes proliferation, invasion and EMT; inhibits apoptosis; promotes tumor growth in vivo in OSCC. |
| Molecular mechanism: |
Acts as a ceRNA that sponges miR-637 to relieve repression of NUPR1, thereby activating the PI3K/AKT pathway. |
| Biological pathway or process: |
PI3K/AKT (promotes); EMT (promotes); proliferation (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Transfection; CCK8; BrdU; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circHIPK3 expression is associated with distant metastasis and higher tumor staging and predicts shorter survival; potential prognostic marker in OSCC. |
| Description: |
circHIPK3 is up-regulated in OSCC and promotes proliferation, invasion and EMT while inhibiting apoptosis. Mechanistically, it sponges miR-637 to upregulate NUPR1 and activate the PI3K/AKT pathway, and high circHIPK3 indicates poorer survival. |
| Confidence score: |
0.8438 |