circRNA basic information
circBase ID: hsa_circ_0003074
Name: hsa_circ_DHTKD1
Synonym: circDHTKD1 / circ_0003074
Host Gene: DHTKD1
Genomic location(hg19): chr10:12123470-12133683:+
Genomic location(hg38): chr10:12081471-12091684:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissue specimens; adjacent noncancerous lung tissue specimens; mouse tumour tissue
Cell lines:

H1299; A549; BEAS-2B
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: EIF4A3, IGF2BP2, PFKL
Associated microRNA: -
Biological function: promotes NSCLC cell proliferation and tumour growth; suppresses apoptosis; promotes glycolysis/glucose metabolism
Molecular mechanism: EIF4A3 upregulates circDHTKD1; circDHTKD1 recruits m6A reader IGF2BP2 to stabilize PFKL mRNA, enhancing glycolysis
Biological pathway or process:

glycolysis (promotes); proliferation (promotes); apoptosis (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; FISH / smFISH; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; Colony Formation Assay; EdU Staining; Western Blot; Actinomycin D / DRB Stability Assay; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

-
Description:

circDHTKD1 (hsa_circ_0003074), derived from DHTKD1, is up-regulated in NSCLC tissues and cell lines and promotes malignant phenotypes and tumor growth. Mechanistically, EIF4A3 increases circDHTKD1, which recruits the m6A reader IGF2BP2 to stabilize PFKL mRNA, thereby enhancing glycolysis.
Confidence score:

0.8267
Other information
Title:

EIF4A3-Induced CircDHTKD1 regulates glycolysis in non-small cell lung cancer via stabilizing PFKL.

Journal: Journal of cellular and molecular medicine
Published: 2024
PubMed ID: 39022816
Study type:

combined biological and clinical study
Data availability: The data generated during the current study are available from the corresponding author on request.
Code availability: -