| Expression pattern: |
UP |
| Associated gene: |
HMGA1, E-cadherin, N-cadherin, PCNA |
| Associated microRNA: |
hsa-miR-296-5p |
| Biological function: |
Regulates EMT and affects proliferation, invasion, and migration of cervical cancer cells. |
| Molecular mechanism: |
Acts as a miRNA sponge for hsa-miR-296-5p, affecting HMGA1 expression and EMT markers (E-cadherin/N-cadherin). |
| Biological pathway or process: |
EMT (other); proliferation (other); migration (other); invasion (other); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Transwell Assay; RNA Pull-Down; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
- |
| Description: |
hsa_circ_0000511 is up-regulated in cervical cancer cell lines and is mainly localized in the cytoplasm. It functions as a ceRNA by sponging hsa-miR-296-5p to modulate HMGA1 and EMT-related markers (E-cadherin/N-cadherin), thereby influencing proliferation, migration, and invasion; the study also validates effects in a nude-mouse xenograft model. |
| Confidence score: |
0.762 |