| Expression pattern: |
UP |
| Associated gene: |
HNF4a, YAP |
| Associated microRNA: |
miR-582-3p |
| Biological function: |
promotes HCC tumorigenesis and malignant phenotypes (cell viability and colony formation) and serves as a serum diagnostic biomarker |
| Molecular mechanism: |
HNF4a transcriptionally activates circ_104075; circ_104075 acts as a ceRNA sponging miR-582-3p to upregulate YAP; m6A modification in YAP 3′UTR is essential for miR-582-3p-YAP interaction |
| Biological pathway or process: |
ceRNA regulation (promotes); Hippo/YAP (promotes); proliferation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; ChIP / ChIP-seq; RIP (RNA Immunoprecipitation); Western Blot; MTT; Colony Formation Assay; ROC Analysis; In Vivo Animal Model |
| Clinical significance: |
Serum circ_104075 showed high diagnostic performance for HCC (AUC-ROC 0.973; sensitivity 96.0%; specificity 98.3%); higher HCC stage associated with higher serum circ_104075; decreased after curative surgery |
| Description: |
circ_104075 is up-regulated in HCC tissues, cell lines, and patient serum. It is transcriptionally induced by HNF4a and promotes HCC tumorigenesis by acting as a ceRNA that sponges miR-582-3p to increase YAP expression, and it shows strong diagnostic value in serum for distinguishing HCC from controls. |
| Confidence score: |
0.8008 |