circRNA basic information
circBase ID: hsa_circ_0000284
Name: hsa_circ_HIPK3
Synonym: circHIPK3
Host Gene: HIPK3
Genomic location(hg19): chr11:33307958-33309057:+
Genomic location(hg38): chr11:33286412-33287511:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BCa tissues; adjacent tissues (ANT); tumor tissues
Cell lines:

MCF-10A; MCF7; SK-BR-3; BT549; BT20; MDA-MB-231; MDA-MB-453
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: Ago2
Associated microRNA: miR-326
Biological function: promotes cell viability, proliferation, migration and invasion; inhibits apoptosis; promotes EMT; promotes tumor growth
Molecular mechanism: circHIPK3 sponges miR-326 (Ago2-dependent) and regulates apoptosis- and EMT-related genes/proteins (e.g., Bcl-2, cleaved Caspase-3, Bax; E-Cadherin, N-Cadherin, Vimentin).
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Nuclear-Cytoplasmic Fractionation; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis
Clinical significance:

circHIPK3 expression was related to tumor size, lymph node metastasis, and TNM stage.
Description:

circHIPK3 (hsa_circ_0000284) is up-regulated in breast cancer tissues/cells and mainly localized in the cytoplasm. It promotes proliferation, migration, invasion, EMT and tumor growth while inhibiting apoptosis, largely by sponging miR-326 (supported by luciferase and Ago2-RIP), thereby modulating apoptosis- and EMT-related proteins (e.g., Bcl-2, Bax, cleaved Caspase-3; E-cadherin, N-cadherin, vimentin).
Confidence score:

0.8175
Other information
Title:

circHIPK3 (hsa_circ_0000284) Promotes Proliferation, Migration and Invasion of Breast Cancer Cells via miR-326.

Journal: OncoTargets and therapy
Published: 2021
PubMed ID: 34135597
Study type:

combined biological and clinical study
Data availability: -
Code availability: -