| Expression pattern: |
UP |
| Associated gene: |
CD44, AGO2 |
| Associated microRNA: |
miR-580-5p |
| Biological function: |
promotes breast cancer cell proliferation and stemness (cancer stem cell properties) |
| Molecular mechanism: |
Exosomal circHIF1A acts as a miRNA sponge for miR-580-5p, relieving repression of CD44. |
| Biological pathway or process: |
stemness (promotes); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
CircHIF1A is upregulated in breast cancer tissues and in blood exosomes from breast cancer patients. |
| Description: |
In breast cancer, exosomal circHIF1A derived from hypoxic CAFs is upregulated and transferred into cancer cells, where it mainly localizes to the cytoplasm. It promotes proliferation and stemness by sponging miR-580-5p and thereby increasing CD44 expression, with supportive in vivo xenograft evidence. |
| Confidence score: |
0.8551 |