| Expression pattern: |
UP |
| Associated gene: |
IGF2BP3, CD44 mRNA, E-cadherin |
| Associated microRNA: |
- |
| Biological function: |
Promotes migration and invasion; promotes EMT by reducing E-cadherin; increases CD44 expression. |
| Molecular mechanism: |
Forms a ternary complex with IGF2BP3 and CD44 mRNA to increase CD44; reduces E-cadherin protein; circFNDC3B can translate an approximately 25 kD peptide. |
| Biological pathway or process: |
migration (promotes); invasion (promotes); EMT (promotes); proliferation (inhibits); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Transfection; Wound Healing Assay; Transwell Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot |
| Clinical significance: |
Ectopic expression of circFNDC3B might serve as a marker of distant metastasis of GC during development and a marker of malignant differentiated cells. |
| Description: |
In gastric cancer cell lines, circFNDC3B promotes migration and invasion and facilitates EMT by reducing E-cadherin protein. Mechanistically, it binds IGF2BP3 and CD44 mRNA to form a ternary complex that elevates CD44 expression, and it also shows protein-coding activity producing an ~25 kD peptide. |
| Confidence score: |
0.74 |