| Expression pattern: |
UP |
| Associated gene: |
TNFAIP8, AGO2 |
| Associated microRNA: |
miR-34a-5p |
| Biological function: |
Promotes DDP resistance and malignant phenotypes (proliferation, migration, invasion) and inhibits apoptosis/cell cycle arrest in DDP-resistant NSCLC; promotes xenograft tumor growth. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-34a-5p, thereby regulating TNFAIP8 expression and DDP-resistant NSCLC progression. |
| Biological pathway or process: |
chemoresistance (promotes); proliferation (promotes); apoptosis (inhibits); cell cycle (other); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
Provides a potential target for DDP-resistant NSCLC diagnosis and treatment. |
| Description: |
circHUWE1 is upregulated in NSCLC, particularly in cisplatin (DDP)-resistant tissues/cells. It promotes DDP resistance and malignant behaviors by sponging miR-34a-5p and derepressing TNFAIP8, and its knockdown suppresses xenograft growth. |
| Confidence score: |
0.8571 |