| Expression pattern: |
UP |
| Associated gene: |
HuR, CCNF |
| Associated microRNA: |
miR-30d, miR-30e |
| Biological function: |
promotes proliferation of LUSC cells and tumor growth |
| Molecular mechanism: |
HuR regulates circPVT1; circPVT1 acts as a ceRNA sponging miR-30d/miR-30e to alleviate their suppression of CCNF |
| Biological pathway or process: |
proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Clinical Sample Validation; Survival Analysis; ROC Analysis; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Colony Formation Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
Higher circPVT1 expression is associated with shorter survival and acts as an independent predictor of OS; potential diagnostic biomarker in tissues and serum (ROC AUC 0.774 and 0.789). |
| Description: |
circPVT1 is up-regulated in LUSC tissues/serum/cell lines and promotes LUSC proliferation and xenograft tumor growth. Mechanistically, HuR upregulates circPVT1, which sponges miR-30d/miR-30e to de-repress CCNF, contributing to poor prognosis and potential diagnostic value. |
| Confidence score: |
0.8226 |