circRNA basic information
circBase ID: hsa_circ_0079929
Name: hsa_circ_CDK13
Synonym: circ-CDK13
Host Gene: CDK13
Genomic location(hg19): chr7:40027197-40027857:+
Genomic location(hg38): chr7:39987598-39988258:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; adjacent normal gastric mucosa tissues; tumor tissues of mice; subcutaneous tumors
Cell lines:

KATO III; MKN-45; KATO/DDP; MKN45/DDP
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: EIF4A3, MDR1, MRP1
Associated microRNA: -
Biological function: Promotes cisplatin resistance, cell viability, tumor growth and tumor cell proliferation, and inhibits cisplatin-induced apoptosis; down-regulation of circ-CDK13 enhances DDP sensitivity in resistant gastric cancer cells, PDO models and mouse xenografts.
Molecular mechanism: Methionine restriction down-regulates EIF4A3, thereby inhibiting circ-CDK13 cyclization and reducing circ-CDK13 levels; reduced circ-CDK13 increases DDP sensitivity and apoptosis, while circ-CDK13 up-regulation increases MDR1 and MRP1 protein expression.
Biological pathway or process:

drug resistance (promotes); chemoresistance (promotes); apoptosis (inhibits); proliferation (promotes); other pathway/process (other)
Detected method:
Q
M
Validation methods:

RT-qPCR; Bioinformatics Analysis; Clinical Sample Validation; Transfection; MTT; Annexin V/PI Flow Cytometry; Western Blot; RNase R Treatment; RNA Pull-Down; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining
Clinical significance:

circ-CDK13 may be a promising prognostic factor and therapeutic target for gastric cancer; circ-CDK13 is elevated in GC clinical samples and DDP-resistant GC samples.
Description:

circ-CDK13 (hsa_circ_0079929) is up-regulated in DDP-resistant gastric cancer cells and clinical GC/DDP-resistant samples. Methionine restriction suppresses EIF4A3-mediated circ-CDK13 cyclization, lowering circ-CDK13 and thereby enhancing cisplatin sensitivity, increasing apoptosis, and reducing tumor growth.
Confidence score:

0.8001
Other information
Title:

Methionine restriction promotes cisplatin sensitivity of gastric cancer resistant cells by down-regulating circ-CDK13 level.

Journal: Experimental cell research
Published: 2024
PubMed ID: 39488295
Study type:

combined biological and clinical study
Data availability: GSE163416; GSE131414; data available from the corresponding author on reasonable request; Supplementary data: https://doi.org/10.1016/j.yexcr.2024.114315
Code availability: -