circRNA basic information
circBase ID: hsa_circ_0010467
Name: hsa_circ_EIF4G3
Synonym: -
Host Gene: EIF4G3
Genomic location(hg19): chr1:21220009-21268823:-
Genomic location(hg38): chr1:20893516-20942330:+
Subcellular localization: nucleus and cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008170
MONDO name: ovarian cancer
Disease details: ovarian cancer
Disease DO ID:
 2394
Disease MeSH ID:
 D010051
Disease NCIt ID:
 C7431
Disease ICD11 ID:
 685124533
Disease OMIM ID:
 167000
Species: Human
Species details: Homo sapiens
Tissue specimen:

OC tissues; serum samples; plasma exosomes; primary tumors; ascites; patient-derived organoids
Cell lines:

SKOV3; A2780; SKOV3-DDP; A2780-DDP; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: LIF, STAT3, AUF1
Associated microRNA: miR-637
Biological function: promotes OC cell proliferation, maintains ovarian cancer stem cell self-renewal and stemness, sustains cisplatin/platinum resistance, and silencing hsa_circ_0010467 increases the anti-tumor efficacy of cisplatin
Molecular mechanism: AUF1 promotes hsa_circ_0010467 biogenesis; hsa_circ_0010467 functions as a ceRNA/miRNA sponge for miR-637, relieves miR-637-mediated repression of LIF, activates LIF/STAT3 signaling, and induces cancer stemness and chemoresistance
Biological pathway or process:

proliferation (promotes); stemness (promotes); chemoresistance (promotes); drug resistance (promotes); apoptosis (inhibits); JAK/STAT (promotes); ceRNA regulation (promotes)
Detected method:
Q
H
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis
Clinical significance:

high expression is positively correlated with advanced tumor stage, poor prognosis, platinum resistance, and may serve as a candidate plasma marker and prognostic biomarker for OC patients treated with platinum-based chemotherapy
Description:

hsa_circ_0010467 is up-regulated in platinum-resistant ovarian cancer tissues, cells, and serum/plasma exosomes, and high expression is associated with advanced tumor stage and poor overall survival. Functionally, it promotes OC cell proliferation, cancer stemness, and cisplatin/platinum resistance. Mechanistically, AUF1 promotes its biogenesis, while hsa_circ_0010467 sponges miR-637 to upregulate LIF and activate STAT3 signaling.
Confidence score:

0.8933
Other information
Title:

AUF1-induced circular RNA hsa_circ_0010467 promotes platinum resistance of ovarian cancer through miR-637/LIF/STAT3 axis.

Journal: Cellular and molecular life sciences : CMLS
Published: 2023
PubMed ID: 37589744
Study type:

combined biological and clinical study
Data availability: GSE214302
Code availability: -