| Expression pattern: |
UP |
| Associated gene: |
SOX4, EZH2, Ago2 |
| Associated microRNA: |
miR-1251 |
| Biological function: |
Promotes PC cell proliferation, EMT, invasiveness and tumor growth; knockdown inhibits these phenotypes. |
| Molecular mechanism: |
Acts as a competing endogenous RNA (miRNA sponge) for miR-1251, leading to increased SOX4 (and EZH2) and promoting EMT/invasion. |
| Biological pathway or process: |
EMT (promotes); invasion (promotes); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Transwell Assay; Western Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Clinical Sample Validation; Cohort Study; Survival Analysis; In Vivo Animal Model |
| Clinical significance: |
High circ_0001666 expression is associated with lymph node metastasis and shorter overall survival/poor prognosis in PC patients. |
| Description: |
circ_0001666 is up-regulated in pancreatic cancer and promotes proliferation, EMT and invasion. Mechanistically, it functions as a miR-1251 sponge to de-repress SOX4 (and increase EZH2), and high circ_0001666 expression is associated with poorer overall survival. |
| Confidence score: |
0.8219 |