circRNA basic information
circBase ID: hsa_circ_0007580
Name: hsa_circ_PRKCA
Synonym: circ-PRKCA
Host Gene: PRKCA
Genomic location(hg19): chr17:64728805-64738878:+
Genomic location(hg38): chr17:66732687-66742760:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissue specimens; adjacent normal tissues; xenograft tumor tissues
Cell lines:

H1650; H1299; H460; A549; 16HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ITGB1
Associated microRNA: miR-384
Biological function: circ-PRKCA promotes NSCLC cell malignancy; curcumin suppresses NSCLC cell viability, colony formation, migration, invasion and xenograft tumor growth by downregulating circ-PRKCA, while promoting apoptosis.
Molecular mechanism: circ-PRKCA functions as a ceRNA by sponging miR-384 to modulate ITGB1 expression in curcumin-treated NSCLC cells.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis
Clinical significance:

-
Description:

hsa_circ_0007580/circ-PRKCA is up-regulated in NSCLC tissues and cells and promotes malignant phenotypes. Curcumin suppresses NSCLC progression by downregulating circ-PRKCA, thereby relieving miR-384-mediated repression of ITGB1 and reducing viability, colony formation, migration, invasion, and xenograft tumor growth while increasing apoptosis.
Confidence score:

0.742
Other information
Title:

Curcumin suppresses the malignancy of non-small cell lung cancer by modulating the circ-PRKCA/miR-384/ITGB1 pathway.

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Published: 2021
PubMed ID: 33684690
Study type:

combined biological and clinical study
Data availability: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request; Supplementary data associated with this article can be found in the online version at doi:10.1016/j.biopha.2021.111439.
Code availability: -