| Expression pattern: |
DN |
| Associated gene: |
ZNF549 |
| Associated microRNA: |
hsa‐miR‐708‐5p, hsa‐miR‐28‐5p |
| Biological function: |
Potential tumor suppressor and diagnostic/dynamic monitoring biomarker in gastric cancer. |
| Molecular mechanism: |
Likely functions via competing endogenous RNA mechanisms (miRNA sponge) based on cytoplasmic enrichment; downstream miRNAs and mRNAs were bioinformatically predicted but not experimentally validated. |
| Biological pathway or process: |
ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Down-regulated in GC serum/tissues/cell lines; serum level associated with tumor differentiation and TNM stage; diagnostic biomarker with AUC 0.745 and improved performance when combined with CEA and CA19-9; postoperative increase suggests utility for dynamic monitoring. |
| Description: |
hsa_circ_0000702 is significantly down-regulated in gastric cancer serum, tissues, and GC cell lines and shows diagnostic utility (AUC 0.745), further improved when combined with CEA and CA19-9. It is enriched slightly in the cytoplasm and is proposed to act via a ceRNA mechanism with predicted miRNAs (hsa-miR-708-5p and hsa-miR-28-5p), supporting its potential as a biomarker for diagnosis and postoperative dynamic monitoring. |
| Confidence score: |
0.6841 |