| Expression pattern: |
UP |
| Associated gene: |
AR, RAB9A |
| Associated microRNA: |
miR-532-3p |
| Biological function: |
circ-LNPEP promotes HCC cell invasion and tumor metastasis under hypoxic conditions and can reverse the suppressive effect of AR on HCC invasion or metastasis. |
| Molecular mechanism: |
AR transcriptionally reduces circ-LNPEP expression; loss of AR increases circ-LNPEP, which acts as a sponge of miR-532e3p and upregulates RAB9A to promote HCC invasion. |
| Biological pathway or process: |
invasion (promotes); metastasis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; RNA Pull-Down; Transfection; Transwell Assay; Luciferase Reporter Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circ-LNPEP may be used as a diagnostic biomarker to predict HCC and may be a therapeutic target for HCC. |
| Description: |
circ-LNPEP is an LNPEP-derived circRNA increased when AR is lost under hypoxia in HCC. It promotes HCC cell invasion and tumor metastasis by sponging miR-532e3p, thereby increasing RAB9A expression, and its overexpression reverses the suppressive effects of AR. The study also suggests circ-LNPEP may have diagnostic and therapeutic relevance in HCC. |
| Confidence score: |
0.7551 |