| Expression pattern: |
UP |
| Associated gene: |
EGFR, ERK, E-cadherin, N-cadherin |
| Associated microRNA: |
miR-7 |
| Biological function: |
promotes migration, invasion, and growth (proliferation) of osteosarcoma cells |
| Molecular mechanism: |
cir-ITCH reduces miR-7 level, thereby increasing EGFR expression and EGFR phosphorylation and activating EGFR/ERK signaling; EGFR phosphorylation is required for cir-ITCH-induced migration and invasion |
| Biological pathway or process: |
ERK (promotes); EGFR (promotes); proliferation (promotes); migration (promotes); invasion (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Transfection; CCK8; Wound Healing Assay; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
cir-ITCH may be a prognostic marker and a promising therapeutic target for osteosarcoma |
| Description: |
In osteosarcoma cell lines, cir-ITCH is up-regulated and promotes proliferation, migration, and invasion. Mechanistically, cir-ITCH suppresses miR-7, resulting in increased EGFR expression and phosphorylation and activation of EGFR/ERK signaling, which drives metastatic phenotypes. |
| Confidence score: |
0.6253 |