| Expression pattern: |
UP |
| Associated gene: |
Sp1, Ago2 |
| Associated microRNA: |
miR-7 |
| Biological function: |
promotes proliferation; promotes migration; promotes invasion; promotes EMT; promotes tumor growth and metastasis |
| Molecular mechanism: |
exosomal circSETDB1 functions as a miRNA sponge for miR-7, thereby upregulating Sp1 and promoting EMT and malignant phenotypes in LUAD |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes); metastasis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis; ROC Analysis; Clinical Sample Validation |
| Clinical significance: |
exosomal circSETDB1 was upregulated in serum exosomes in LUAD patients and associated with disease stage / T stage and lymph node metastasis; ROC AUC=0.601 for LUAD diagnosis |
| Description: |
In LUAD, exosomal circSETDB1 is upregulated under hypoxia and in patient serum exosomes. It promotes proliferation, migration, invasion and EMT by sponging miR-7 to upregulate Sp1, enhancing tumor growth and metastasis. |
| Confidence score: |
0.8871 |