| Expression pattern: |
UP |
| Associated gene: |
PKM2, Ago2, RISC |
| Associated microRNA: |
miR-326-3p, miR-330-5p |
| Biological function: |
promotes aerobic glycolysis and proliferation (increases cell viability, glucose uptake and lactate production) in gastric cancer cells |
| Molecular mechanism: |
circATP2B1 acts as a ceRNA/miRNA sponge for the miR-326 gene cluster (miR-326-3p and miR-330-5p) in an Ago2/RISC-dependent manner, relieving repression of PKM2 and promoting aerobic glycolysis |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Actinomycin D / DRB Stability Assay; Transfection; CCK8; EdU Staining; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Clinical Sample Validation |
| Clinical significance: |
patients who had higher circATP2B1 expression had a poorer prognosis; progression free survival and overall survival were shorter in the higher circATP2B1 group |
| Description: |
circATP2B1 is up-regulated in gastric cancer tissues and cell lines and mainly localizes to the cytoplasm. It promotes aerobic glycolysis and proliferation by sponging miR-326-3p/miR-330-5p (miR-326 gene cluster) in an Ago2/RISC-dependent manner, thereby relieving repression of PKM2. |
| Confidence score: |
0.8606 |