| Expression pattern: |
UP |
| Associated gene: |
STX6 / Syntaxin 6, Ago2 |
| Associated microRNA: |
miR-198 |
| Biological function: |
Promotes osteosarcoma progression by enhancing proliferation, migration and invasion, facilitating cell cycle progression, and inhibiting apoptosis; supports xenograft tumor growth. |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-198 to upregulate STX6 (Syntaxin 6). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Cohort Study; Survival Analysis; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; Western Blot; IHC (Immunohistochemistry) |
| Clinical significance: |
High circ_0010220 expression is associated with lower survival rate (poor overall survival) in osteosarcoma patients. |
| Description: |
circ_0010220 (from ARHGEF10L) is up-regulated in osteosarcoma tissues/cells and its high expression is linked to poorer survival. Functionally, circ_0010220 promotes malignant phenotypes and xenograft growth by sponging miR-198 to derepress STX6, forming a circ_0010220/miR-198/STX6 ceRNA axis. |
| Confidence score: |
0.8621 |