| Expression pattern: |
UP |
| Associated gene: |
FZD3, p-beta-catenin, GPX4, SLC7A11, P-gp, MRP1 |
| Associated microRNA: |
miR-30a-5p |
| Biological function: |
Inhibits 5-FU chemosensitivity (promotes chemoresistance), and suppresses ferroptosis; promotes viability, migration and invasion in 5-FU-resistant ESCC cells. |
| Molecular mechanism: |
ceRNA/miRNA sponge mechanism: circPVT1 sponges miR-30a-5p to increase FZD3, affecting Wnt/beta-catenin signaling and ferroptosis markers (GPX4, SLC7A11). |
| Biological pathway or process: |
Wnt/beta-catenin (inhibits); ferroptosis (promotes); chemoresistance (inhibits); migration (inhibits); invasion (inhibits); proliferation (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
circPVT1 is upregulated in 5-FU-resistant ESCC cells and promotes chemoresistance. Mechanistically, circPVT1 sponges miR-30a-5p to upregulate FZD3, activating Wnt/beta-catenin signaling and suppressing ferroptosis (via GPX4/SLC7A11), thereby reducing 5-FU chemosensitivity. |
| Confidence score: |
0.6287 |