| Expression pattern: |
UP |
| Associated gene: |
AGO2 |
| Associated microRNA: |
miR-582-3p |
| Biological function: |
promotes proliferation, migration and invasion; inhibits apoptosis in HCC cells |
| Molecular mechanism: |
Acts as a sponge for miR-582-3p, negatively regulating miR-582-3p and affecting downstream proliferation/metastasis and apoptosis markers (cyclin D1, MMP-9, Bcl-2, Bax). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circRNA_PTPRA/miR-582-3p axis may represent a potential target for HCC diagnosis and treatment. |
| Description: |
circRNA_PTPRA is upregulated in HCC cell lines and promotes malignant phenotypes by sponging miR-582-3p. Silencing circRNA_PTPRA increases miR-582-3p and suppresses proliferation, migration and invasion while promoting apoptosis, with rescue by miR-582-3p inhibition. |
| Confidence score: |
0.6253 |