| Expression pattern: |
UP |
| Associated gene: |
eIF3a, AGO2 |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation, migration, and invasion of NSCLC cells |
| Molecular mechanism: |
regulates the expression of its parental/host gene eIF3a (not via AGO2-associated miRNA sponge) |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); EMT (inhibits); PI3K/AKT (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot |
| Clinical significance: |
- |
| Description: |
hsa_circ_0092856 is up-regulated in NSCLC cell lines and promotes proliferation, migration, and invasion. It localizes mainly to the cytoplasm and appears not to act as an AGO2-associated miRNA sponge; instead, it enhances NSCLC progression by up-regulating its host/parental gene eIF3a (with associated changes such as increased p-Akt and BCL2 and reduced BAX and E-cadherin upon overexpression). |
| Confidence score: |
0.6649 |