| Expression pattern: |
DN |
| Associated gene: |
SRSF1, beta-catenin, E-cadherin |
| Associated microRNA: |
- |
| Biological function: |
Reverses oxLDL-induced inhibition of HUVEC proliferation; inhibits oxLDL-induced apoptosis and migration; improves tube formation; relieves lipid accumulation and mitochondrial injury in oxLDL-treated HUVECs. |
| Molecular mechanism: |
Binds to SRSF1 and reverses oxLDL-induced activation of beta-catenin; proposed SRSF1/beta-catenin/EMT axis involvement. |
| Biological pathway or process: |
Wnt/beta-catenin (inhibits); proliferation (promotes); apoptosis (inhibits); migration (inhibits); angiogenesis (promotes); mitochondrial function (promotes); EMT (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNA Pull-Down; Western Blot; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Oil Red O Staining |
| Clinical significance: |
- |
| Description: |
In an in vitro atherosclerosis model (oxLDL-treated HUVECs), hsa_circ_0001445 is down-regulated, and its overexpression counteracts oxLDL-induced endothelial dysfunction by promoting proliferation and reducing apoptosis and migration. Mechanistically, hsa_circ_0001445 binds the RBP SRSF1 and suppresses beta-catenin/Wnt signaling activity, consistent with an SRSF1/beta-catenin/EMT-related axis. |
| Confidence score: |
0.5312 |