| Expression pattern: |
UP |
| Associated gene: |
CDK16, JAK2, STAT3 |
| Associated microRNA: |
miR-324-5p |
| Biological function: |
Promotes HCC malignant behaviors (cell growth/proliferation, cell cycle progression, migration, invasion) and inhibits apoptosis; GL63 exerts anti-tumor effects partly by downregulating circZNF83. |
| Molecular mechanism: |
Acts as a ceRNA sponge for miR-324-5p to derepress CDK16, thereby affecting JAK2/STAT3 signaling; GL63 downregulates circZNF83 to inhibit this axis. |
| Biological pathway or process: |
JAK/STAT (inhibits); proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Transwell Assay; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
circZNF83 is up-regulated in HCC tissues and cell lines and functions as an oncogenic circRNA. It sponges miR-324-5p to increase CDK16 expression and support malignant phenotypes, and GL63 suppresses HCC partly by downregulating circZNF83 to inhibit the miR-324-5p/CDK16 axis and inactivate JAK2/STAT3 signaling. |
| Confidence score: |
0.7052 |