circRNA basic information
circBase ID: -
Name: hsa_circ_PVT1
Synonym: circPVT1
Host Gene: PVT1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

osteosarcoma tissues; normal bone tissue; tumor tissues (xenografts)
Cell lines:

KHOS; U2OS; KHOS/DXR; U2OS/DXR; hFOB1.19; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: TRIAP1
Associated microRNA: miR-137
Biological function: promotes doxorubicin resistance and tumor growth; promotes proliferation/viability and inhibits apoptosis in DXR-resistant osteosarcoma cells
Molecular mechanism: ceRNA/miRNA sponge mechanism: circPVT1 sponges miR-137 to upregulate TRIAP1
Biological pathway or process:

ceRNA regulation (promotes); drug resistance (promotes); proliferation (promotes); apoptosis (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model
Clinical significance:

circPVT1 was apparently higher in the chemoresistant group compared with the chemosensitive group
Description:

circPVT1 is up-regulated in osteosarcoma, especially in doxorubicin-resistant tissues/cells, and promotes DXR resistance. Mechanistically, it sponges miR-137 to upregulate TRIAP1, enhancing cell survival/proliferation and reducing apoptosis, and it supports resistant tumor growth in a xenograft model.
Confidence score:

0.7045
Other information
Title:

Circular RNA circPVT1 Contributes to Doxorubicin (DXR) Resistance of Osteosarcoma Cells by Regulating TRIAP1 via miR-137.

Journal: BioMed research international
Published: 2021
PubMed ID: 33981772
Study type:

combined biological and clinical study
Data availability: Please contact the correspondence author for the data request.
Code availability: -