| Expression pattern: |
UP |
| Associated gene: |
GSDMD, caspase-1, caspase-11, IL-1beta, IL-6, IL-8, TNF-alpha |
| Associated microRNA: |
miR-193a-5p |
| Biological function: |
Promotes acinar cell pyroptosis and inflammatory factor release; aggravates acute pancreatitis progression. |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA-like): circHIPK3 binds and suppresses miR-193a-5p, thereby derepressing GSDMD to enhance caspase-1/11-associated pyroptosis. |
| Biological pathway or process: |
pyroptosis (promotes); inflammation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Transfection; Luciferase Reporter Assay; Western Blot; ELISA; CCK8; Flow Cytometry（Non-apoptosis/cycle） |
| Clinical significance: |
circHIPK3 may be a useful biomarker for AP diagnosis and a promising therapeutic target for AP treatment. |
| Description: |
circHIPK3 is up-regulated in AP patient serum and in caerulein-stimulated AR42J acinar cells. It promotes inflammatory injury and pyroptosis by sponging miR-193a-5p to increase GSDMD expression, leading to caspase-1/11 activation and cytokine release; it is proposed as a diagnostic biomarker and therapeutic target for AP. |
| Confidence score: |
0.6152 |