| Expression pattern: |
DN |
| Associated gene: |
PDCD4 |
| Associated microRNA: |
miR-106b-5p |
| Biological function: |
inhibits migration and invasion (metastasis) of ccRCC cells; suppresses tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circ-ITCH sponges miR-106b-5p to increase PDCD4 expression, inhibiting metastasis-related behaviors |
| Biological pathway or process: |
migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
circ-ITCH may function as a novel diagnostic target to suppress ccRCC metastasis. |
| Description: |
circ-ITCH is down-regulated in ccRCC tissues/cell lines and acts as a tumor suppressor by inhibiting migration and invasion. Mechanistically, it sponges miR-106b-5p to relieve repression of PDCD4, thereby suppressing ccRCC metastasis and reducing xenograft tumor growth. |
| Confidence score: |
0.6261 |