circRNA basic information
circBase ID: hsa_circ_0000936
Name: hsa_circ_SHKBP1
Synonym: circSHKBP1
Host Gene: SHKBP1
Genomic location(hg19): chr19:41089303-41089623:+
Genomic location(hg38): chr19:40576873-40591397:.
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; adjacent normal tissues; serum; serum exosomes; xenograft tumors; lung metastatic lesions
Cell lines:

BGC823; HGC27; AGS; MGC803; GES1; HEK-293 T; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HUR, VEGF, HSP90, STUB1
Associated microRNA: miR-582-3p
Biological function: promotes proliferation, migration, invasion and angiogenesis; promotes tumor growth and metastasis
Molecular mechanism: ceRNA sponging miR-582-3p to increase HUR and enhance VEGF mRNA stability; directly binds HSP90 and blocks STUB1-HSP90 interaction to inhibit HSP90 ubiquitination and degradation
Biological pathway or process:

ceRNA regulation (promotes); angiogenesis (promotes); mRNA stability (promotes); ubiquitination (inhibits); metastasis (promotes); proliferation (promotes); migration (promotes); invasion (promotes)
Detected method:
Q
H
S
Validation methods:

RNA-seq; RT-qPCR; ISH (In Situ Hybridization); RNase R Treatment; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Western Blot; CCK8; EdU Staining; Transwell Assay; Tube Formation Assay; ELISA; Co-IP; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Increased expression of circSHKBP1 in GC tissues and serum was related to advanced TNM stage and poor survival; circSHKBP1 decreased after gastrectomy; promising circulating biomarker for GC diagnosis and prognosis
Description:

circSHKBP1 (hsa_circ_0000936) is up-regulated in gastric cancer tissues and serum exosomes and is associated with advanced TNM stage and poor survival. Functionally, it promotes GC cell proliferation, migration, invasion, angiogenesis, tumor growth and metastasis. Mechanistically, it sponges miR-582-3p to increase HUR and stabilize/raise VEGF, and it also binds HSP90 to block STUB1-mediated ubiquitination, reducing HSP90 degradation; exosomal circSHKBP1 supports intercellular communication and is proposed as a circulating biomarker.
Confidence score:

0.8846
Other information
Title:

Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation.

Journal: Molecular cancer
Published: 2020
PubMed ID: 32600329
Study type:

combined biological and clinical study
Data availability: 10.1186/s12943-020-01208-3; Additional file 1; Additional file 2; Additional file 3
Code availability: -