circRNA basic information
circBase ID: hsa_circ_0008305
Name: hsa_circ_PTK2
Synonym: circPTK2
Host Gene: PTK2
Genomic location(hg19): chr8:141799572-141840625:-
Genomic location(hg38): chr8:140789473-140830526:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005229
MONDO name: bacterial infectious disease with sepsis
Disease details: sepsis
Disease DO ID:
 0040085
Disease MeSH ID:
 D016470
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

mouse hippocampus
Cell lines:

BV2
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: HMGB1
Associated microRNA: miR-181c-5p
Biological function: promotes microglia activation/inflammation and hippocampal neuronal (hippocampal cell) apoptosis in sepsis models; circPTK2 knockdown improves cognitive function and increases survival in CLP-induced mice
Molecular mechanism: circPTK2 acts as a ceRNA to target/negatively regulate miR-181c-5p, thereby relieving miR-181c-5p-mediated repression of HMGB1 and promoting inflammatory cytokine production and apoptosis
Biological pathway or process:

inflammation (promotes); apoptosis (promotes); mitochondrial function (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; TUNEL; In Vivo Animal Model; Western Blot; ELISA; Survival Analysis
Clinical significance:

circPTK2 could be the target in therapeutics for sepsis-induced cognitive dysfunction
Description:

circPTK2 (hsa_circ_0008305) is up-regulated in LPS-stimulated microglia and in the hippocampus of CLP-induced sepsis mice. It promotes microglial inflammatory activation and hippocampal cell apoptosis by sponging miR-181c-5p, which in turn targets HMGB1. Silencing circPTK2 alleviates inflammation, improves mitochondrial membrane potential and cognitive performance, and increases survival in septic mice.
Confidence score:

0.7164
Other information
Title:

CircPTK2-miR-181c-5p-HMGB1: a new regulatory pathway for microglia activation and hippocampal neuronal apoptosis induced by sepsis.

Journal: Molecular medicine (Cambridge, Mass.)
Published: 2021
PubMed ID: 33952191
Study type:

combined biological and clinical study
Data availability: The analyzed data sets generated during the study are available from the corresponding author on reasonable request.
Code availability: -