| Expression pattern: |
UP |
| Associated gene: |
CXCL12 |
| Associated microRNA: |
miR-182-5p |
| Biological function: |
Promotes ox-LDL-induced HUVEC injuries (apoptosis, oxidative stress, inflammation) and counteracts the protective effects of atorvastatin; its knockdown is protective. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-182-5p, thereby relieving miR-182-5p-mediated repression of CXCL12 and increasing CXCL12 expression in ox-LDL/ATV-treated HUVECs. |
| Biological pathway or process: |
apoptosis (promotes); cell cycle (inhibits); inflammation (promotes); oxidative phosphorylation (unclear); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; ELISA; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
In an ox-LDL-induced HUVEC injury model of atherosclerosis, hsa_circ_0004831 is increased by ox-LDL and reduced by atorvastatin. Functionally, hsa_circ_0004831 promotes endothelial injury phenotypes (apoptosis, oxidative stress, inflammation) and attenuates atorvastatin protection by sponging miR-182-5p to upregulate CXCL12. |
| Confidence score: |
0.7822 |