| Expression pattern: |
UP |
| Associated gene: |
fibronectin 1 / FN1, MMP2, MMP9, Ago2 |
| Associated microRNA: |
miR-615-5p |
| Biological function: |
promotes migration, invasion and metastasis (without affecting proliferation) in LUAD |
| Molecular mechanism: |
acts as a miRNA sponge for miR-615-5p to upregulate fibronectin 1, thereby increasing MMP2 and MMP9 expression and promoting LUAD metastasis |
| Biological pathway or process: |
migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Western Blot; IHC (Immunohistochemistry) |
| Clinical significance: |
Increased circ-CAMK2A expression was closely associated with lymph node metastasis, distant metastasis, advanced TNM stage and unfavorable outcome; circ-CAMK2A was an independent risk prognostic factor for overall survival of LUAD patients. |
| Description: |
circ-CAMK2A is up-regulated in LUAD and promotes migration, invasion, and lung metastasis. It mainly acts as a ceRNA by sponging miR-615-5p to increase FN1 expression and consequently elevate MMP2/MMP9, and high circ-CAMK2A is associated with poor prognosis. |
| Confidence score: |
0.8262 |