circRNA basic information
circBase ID: hsa_circ_0001821
Name: hsa_circ_PVT1
Synonym: CircPVT1
Host Gene: PVT1
Genomic location(hg19): chr8:128902834-128903244:+
Genomic location(hg38): chr8:127890588-127890998:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008903
MONDO name: lung cancer
Disease details: lung cancer
Disease DO ID:
 1324
Disease MeSH ID:
 -
Disease NCIt ID:
 C7377
Disease ICD11 ID:
 -
Disease OMIM ID:
 211980
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

A549; HCT116
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UN
Associated gene: NRAS, KRAS, MYC, HMGA2
Associated microRNA: let-7, hsa-let-7a-5p
Biological function: Promotes proliferation and migration, confers resistance to apoptosis, and drives EMT/oncogenic phenotypes in cancer cells; maintains/upregulates NRAS expression.
Molecular mechanism: circPVT1 derepresses NRAS by sponging/sequestering let-7, reducing let-7 binding to the NRAS 3′UTR; rescue by NRAS let-7 target protector supports a circPVT1/let-7/NRAS ceRNA-like mechanism (availability change rather than let-7 degradation).
Biological pathway or process:

proliferation (promotes); migration (promotes); apoptosis (inhibits); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Transfection; Luciferase Reporter Assay; Western Blot; EdU Staining; Annexin V/PI Flow Cytometry; Wound Healing Assay; IF (Immunofluorescence)
Clinical significance:

-
Description:

In A549 lung cancer and HCT116 colorectal cancer cells, circPVT1 promotes oncogenic phenotypes (proliferation, migration, apoptosis resistance and EMT). Mechanistically, circPVT1 antagonizes let-7 to relieve repression of NRAS via the NRAS 3′UTR, and blocking let-7/NRAS binding rescues NRAS reduction and several phenotypes caused by circPVT1 knockdown.
Confidence score:

0.6733
Other information
Title:

CircPVT1 attenuates negative regulation of NRAS by let-7 and drives cancer cells towards oncogenicity.

Journal: Scientific reports
Published: 2021
PubMed ID: 33907219
Study type:

biological research
Data availability: All data generated or analysed during the current study are available from the corresponding author on reasonable request.
Code availability: -