circRNA basic information
circBase ID: hsa_circ_0004104
Name: hsa_circ_SPARC
Synonym: circ_0004104
Host Gene: SPARC
Genomic location(hg19): chr5:151043647-151049345:-
Genomic location(hg38): chr5:151664086-151669784:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005311
MONDO name: atherosclerosis
Disease details: atherosclerosis
Disease DO ID:
 1936
Disease MeSH ID:
 D050197
Disease NCIt ID:
 C35768
Disease ICD11 ID:
 109367356
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

HUVECs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: TRIM14
Associated microRNA: miR-328-3p
Biological function: Promotes ox-LDL-induced endothelial dysfunction by decreasing viability and angiogenesis and increasing apoptosis, inflammation and oxidative stress in HUVECs.
Molecular mechanism: Acts as a miRNA sponge for miR-328-3p, thereby positively regulating TRIM14 expression.
Biological pathway or process:

apoptosis (promotes); angiogenesis (inhibits); inflammation (promotes); other pathway/process (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; MTT; Annexin V/PI Flow Cytometry; Tube Formation Assay; ELISA; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Bioinformatics Analysis
Clinical significance:

-
Description:

In an ox-LDL-induced HUVEC atherosclerosis cell model, circ_0004104 is up-regulated and exacerbates endothelial injury (reduced viability/angiogenesis; increased apoptosis, inflammation, and oxidative stress). Mechanistically, circ_0004104 functions as a ceRNA by sponging miR-328-3p to up-regulate TRIM14, and circ_0004104 knockdown alleviates these dysfunctions.
Confidence score:

0.762
Other information
Title:

Circ_0004104 knockdown alleviates oxidized low-density lipoprotein-induced dysfunction in vascular endothelial cells through targeting miR-328-3p/TRIM14 axis in atherosclerosis.

Journal: BMC cardiovascular disorders
Published: 2021
PubMed ID: 33892646
Study type:

biological research
Data availability: The online version contains supplementary material available at 10.1186/s12872-021-02012-7.
Code availability: -