circRNA basic information
circBase ID: -
Name: hsa_circ_SCMH1
Synonym: circSCMH1
Host Gene: SCMH1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005266
MONDO name: diabetic retinopathy
Disease details: Diabetic retinopathy
Disease DO ID:
 8947
Disease MeSH ID:
 D003930
Disease NCIt ID:
 C34538
Disease ICD11 ID:
 1006882070
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

retina; retinal tissues
Cell lines:

ARPE-19
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: ZEB1
Associated microRNA: miR-200a-3p
Biological function: Promotes high glucose-induced ARPE-19 cell proliferation, migration, and EMT; suppresses apoptosis; knockdown attenuates EMT in the retina of diabetic mice.
Molecular mechanism: circSCMH1 acts as a miR-200a-3p sponge and positively regulates ZEB1 expression, thereby inducing high glucose-treated ARPE-19 cell growth and EMT.
Biological pathway or process:

proliferation (promotes); migration (promotes); EMT (promotes); apoptosis (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; TUNEL; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis
Clinical significance:

The circSCMH1/miR-200a-3p/ZEB1 axis may provide novel therapeutic targets for DR treatment.
Description:

circSCMH1 is up-regulated in high glucose-stimulated human ARPE-19 retinal pigment epithelial cells and promotes diabetic retinopathy-related cell proliferation, migration, and EMT while inhibiting apoptosis. Mechanistically, circSCMH1 sponges miR-200a-3p to activate ZEB1 expression, and circSCMH1 knockdown alleviates retinal EMT in STZ-induced diabetic mice.
Confidence score:

0.6594
Other information
Title:

CircRNA SCMH1 regulates the miR-200a-3p/ZEB1 signaling axis to promote diabetes-induced retinal epithelial-mesenchymal transition.

Journal: Experimental eye research
Published: 2022
PubMed ID: 36162459
Study type:

biological research
Data availability: All data generated or analyzed during this study are included in this article; The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request; No data was used for the research described in the article.
Code availability: -