circRNA basic information
circBase ID: hsa_circ_0000043
Name: hsa_circ_PUM1
Synonym: circPUM1
Host Gene: PUM1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tumours
Cell lines:

HCCLM3; MHCC97H; MHCC97L; SK‐HEP‐1; HUH7; HEPG2; THLE‐3
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UN
Associated gene: MAP3K2, AGO2
Associated microRNA: miR-1208
Biological function: Promotes HCC cell proliferation, migration, invasion, and EMT; circPUM1 knockdown suppresses tumor growth in vivo.
Molecular mechanism: Acts as a miRNA sponge for miR-1208 to up-regulate MAP3K2, promoting HCC progression and EMT.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model
Clinical significance:

-
Description:

circPUM1 functions as an oncogenic circRNA in HCC, promoting proliferation, migration, invasion and EMT. Mechanistically, it sponges miR-1208 to de-repress MAP3K2, and circPUM1 knockdown suppresses xenograft tumor growth with reduced EMT markers.
Confidence score:

0.7002
Other information
Title:

CircPUM1 promotes hepatocellular carcinoma progression through the miR-1208/MAP3K2 axis.

Journal: Journal of cellular and molecular medicine
Published: 2021
PubMed ID: 33320435
Study type:

biological research
Data availability: The data used to support the findings of this study are available from the corresponding author upon request.
Code availability: -