| Expression pattern: |
UP |
| Associated gene: |
STAT3 |
| Associated microRNA: |
miR-125b |
| Biological function: |
promotes cell proliferation |
| Molecular mechanism: |
circPVT1 acts as a competing endogenous RNA by sponging miR-125b, thereby releasing STAT3 from miR-125b-mediated repression and increasing STAT3 to promote proliferation. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); cell cycle (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Cell Cycle Assay; IF (Immunofluorescence); Western Blot; Clinical Sample Validation; ROC Analysis |
| Clinical significance: |
circPVT1 showed diagnostic performance in 50 paired OSCC tissues (AUC=0.787) and its expression was positively correlated with tumor size and TNM stage. |
| Description: |
circPVT1 is up-regulated in OSCC tissues and cell lines and promotes OSCC cell proliferation. It mainly localizes in the cytoplasm and functions as a ceRNA by sponging miR-125b, thereby increasing STAT3 expression and driving proliferative phenotypes. |
| Confidence score: |
0.7845 |