circRNA basic information
circBase ID: hsa_circ_0031242
Name: hsa_circ_PRMT5
Synonym: circ_0031242
Host Gene: PRMT5
Genomic location(hg19): chr14:23389732-23392044:-
Genomic location(hg38): chr14:22920523-22922835:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor specimens; adjacent noncancerous hepatic tissues

Cell lines:

THLE-2; Huh7; SNU-387; Huh7-R; SNU-387-R

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: POU3F2, Argonaute2, Wnt/beta-catenin, c-myc
Associated microRNA: miR-924
Biological function: promotes DDP resistance and malignant progression (viability, migration, invasion) and inhibits apoptosis in DDP-resistant HCC cells
Molecular mechanism: ceRNA sponge of miR-924 to upregulate POU3F2, thereby promoting cisplatin resistance and progression
Biological pathway or process:

chemoresistance (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); Wnt/beta-catenin (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model

Clinical significance:

circ_0031242 was higher in recurrent HCC patients after DDP-based chemotherapy than in primary (DDP-sensitive) patients, and proposed as a potential therapeutic target for chemoresistant HCC

Description:

circ_0031242 (circ_PRMT5) is up-regulated in HCC, especially in DDP-resistant recurrent tumors and DDP-resistant cell lines. It promotes cisplatin resistance and malignant phenotypes by sponging miR-924 to increase POU3F2 expression, and its knockdown sensitizes xenografts to DDP.

Confidence score:

0.6825

Other information
Title:

Circ_0031242 Silencing Mitigates the Progression and Drug Resistance in DDP-Resistant Hepatoma Cells by the miR-924/POU3F2 Axis.

Journal: Cancer management and research
Published: 2021
PubMed ID: 33531841
Study type:

combined biological and clinical study

Data availability: -
Code availability: -