| Expression pattern: |
UP |
| Associated gene: |
444 mRNA |
| Associated microRNA: |
hsa-miR-145-5p, hsa-miR-240-5p, hsa-miR-211-5p |
| Biological function: |
promotes trophoblast proliferation, migration, and invasion under high glucose; knockdown inhibits these functions |
| Molecular mechanism: |
predicted ceRNA network with 45 miRNA binding sites and 444 mRNAs; may act via PI3K/Akt signaling pathway |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); PI3K/AKT (promotes); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Clinical Sample Validation; Bioinformatics Analysis; Transfection; MTT; Transwell Assay; EdU Staining |
| Clinical significance: |
Upregulated in GDM plasma and correlated with total cholesterol and LDL-C; may be a potentially sensitive serum biomarker for GDM |
| Description: |
circ_0008285 is upregulated in GDM patient plasma and in high glucose-treated HTR-8/SVneo trophoblast cells. Silencing circ_0008285 suppresses trophoblast proliferation, migration, and invasion under high glucose. Mechanistically, the study predicts a circ_0008285-miRNA-mRNA network and suggests involvement of PI3K/AKT signaling, supporting its potential as a serum biomarker/therapeutic target clue in GDM. |
| Confidence score: |
0.7477 |