| Expression pattern: |
UP |
| Associated gene: |
PTEN, Akt |
| Associated microRNA: |
miR-221-3p |
| Biological function: |
Suppresses trophoblast cell migration, invasion and EMT (circVRK1 knockdown promotes these phenotypes), contributing to PE progression. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-221-3p to regulate PTEN and thereby modulate Akt phosphorylation (PI3K/Akt signaling). |
| Biological pathway or process: |
migration (inhibits); invasion (inhibits); EMT (inhibits); PI3K/AKT (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Sanger Sequencing; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; FISH / smFISH; RNA Pull-Down; circRNA-seq; Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; IF (Immunofluorescence); Western Blot; Clinical Sample Validation |
| Clinical significance: |
circVRK1 serves as a potential biomarker for the diagnosis of PE. |
| Description: |
circVRK1 (hsa_circ_0000566), derived from VRK1, is up-regulated in pre-eclampsia placental tissues. It localizes mainly in the cytoplasm and suppresses trophoblast migration, invasion and EMT by sponging miR-221-3p to derepress PTEN and reduce Akt phosphorylation/PI3K-Akt activation. |
| Confidence score: |
0.8405 |