| Expression pattern: |
UP |
| Associated gene: |
STAT3, HK2 |
| Associated microRNA: |
miR-515-5p |
| Biological function: |
Promotes GC cell proliferation and enhances the Warburg effect (increased glucose consumption, lactate production, ATP quantity, and ECAR; reduced OCR); knockdown represses tumor growth in vivo. |
| Molecular mechanism: |
circCUL3 acts as a ceRNA (miRNA sponge) for miR-515-5p to upregulate STAT3; STAT3 transcriptionally activates HK2 by binding the HK2 promoter, promoting aerobic glycolysis (Warburg effect). |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); other pathway/process (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Western Blot; ChIP / ChIP-seq; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis; Clinical Sample Validation; Cohort Study |
| Clinical significance: |
High circCUL3 expression correlates with advanced clinical stage and unfavorable prognosis/overall survival in GC patients. |
| Description: |
circCUL3 is up-regulated in gastric cancer tissues and cell lines and is associated with worse overall survival. It promotes GC cell proliferation and aerobic glycolysis (Warburg effect) by sponging miR-515-5p to increase STAT3, which then transcriptionally upregulates HK2; circCUL3 knockdown suppresses xenograft tumor growth. |
| Confidence score: |
0.8784 |