| Expression pattern: |
UP |
| Associated gene: |
CDK1, AGO2, METTL3, METTL14, FTO, m6A |
| Associated microRNA: |
miR-31-5p |
| Biological function: |
promotes cell proliferation, migration and invasion; facilitates tumorigenesis in vivo |
| Molecular mechanism: |
Acts as a ceRNA (miRNA sponge) for miR-31-5p to upregulate CDK1; its expression is promoted by m6A modification in an METTL3-dependent manner |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes); m6A modification (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Transwell Assay; Wound Healing Assay; Western Blot; MeRIP / MeRIP-seq; Clinical Sample Validation; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
may serve as a potential biomarker and therapeutic target for breast cancer patients |
| Description: |
circMETTL3 is up-regulated in breast cancer tissues/cell lines and promotes proliferation, migration, invasion and xenograft tumor growth. It mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-31-5p to increase CDK1 expression; its expression is enhanced by m6A modification and is positively regulated by its host gene METTL3 in an m6A-dependent manner. |
| Confidence score: |
0.8784 |