| Expression pattern: |
DN |
| Associated gene: |
eIF4A3, beclin1 mRNA, FOXO1 mRNA, AGO2 |
| Associated microRNA: |
- |
| Biological function: |
Inhibits ox-LDL-induced abnormal autophagy and endothelial cell apoptosis; increases atherosclerotic plaque stability. |
| Molecular mechanism: |
Acts as a protein sponge for eIF4A3, blocking eIF4A3 recruitment to beclin1 and FOXO1 mRNAs and decreasing their mRNA stability/expression. |
| Biological pathway or process: |
autophagy (inhibits); apoptosis (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
B
H
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; divergent primers PCR; Sanger Sequencing; Northern Blot; FISH / smFISH; RIP (RNA Immunoprecipitation); Bioinformatics Analysis; Transfection; Actinomycin D / DRB Stability Assay; Western Blot; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Annexin V/PI Flow Cytometry; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Oil Red O Staining; ELISA; Clinical Sample Validation |
| Clinical significance: |
Potential CHD biomarker (PBMC sequencing and plaque-tissue validation) for predicting CHD. |
| Description: |
hsa_circ_0030042 is down-regulated in CHD and atherosclerotic plaques. It suppresses ox-LDL-induced abnormal autophagy and endothelial apoptosis, thereby improving plaque stability. Mechanistically, it acts as a cytoplasmic protein sponge for eIF4A3, reducing eIF4A3 recruitment to beclin1 and FOXO1 mRNAs and lowering their stability/expression. |
| Confidence score: |
0.8488 |