circRNA basic information
circBase ID: -
Name: hsa_circ_HIPK3
Synonym: -
Host Gene: HIPK3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0015924
MONDO name: pulmonary arterial hypertension
Disease details: pulmonary arterial hypertension
Disease DO ID:
 -
Disease MeSH ID:
 D000081029
Disease NCIt ID:
 -
Disease ICD11 ID:
 1931148955
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

hPAECs; 293T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: STAT3, AGO2
Associated microRNA: miR-328-3p
Biological function: promotes proliferation, migration, and angiogenesis (tube formation) of hPAECs in a PAH cell model
Molecular mechanism: ceRNA/miRNA sponge: circHIPK3 binds miR-328-3p and indirectly upregulates STAT3 (protein) in hPAECs
Biological pathway or process:

proliferation (promotes); migration (promotes); angiogenesis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Transwell Assay; Wound Healing Assay; Tube Formation Assay; Western Blot; Bioinformatics Analysis
Clinical significance:

-
Description:

In a PDGF-stimulated hPAEC PAH cell model, circHIPK3 is up-regulated and promotes endothelial proliferation, migration, and tube formation. It acts as a miRNA sponge for miR-328-3p, relieving repression on STAT3 and increasing STAT3 protein levels, thereby contributing to PAH-related vascular remodeling phenotypes.
Confidence score:

0.7406
Other information
Title:

Circular RNA-HIPK3 regulates human pulmonary artery endothelial cells function and vessel growth by regulating microRNA-328-3p/STAT3 axis.

Journal: Pulmonary circulation
Published: 2021
PubMed ID: 33854768
Study type:

biological research
Data availability: -
Code availability: -