| Expression pattern: |
UN |
| Associated gene: |
BIRC5, Ago2 |
| Associated microRNA: |
miR-497-5p |
| Biological function: |
promotes tumorigenesis, cell growth/proliferation and invasion |
| Molecular mechanism: |
Acts as a miR-497-5p sponge to upregulate BIRC5 expression (ceRNA-like regulation). |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); circRIP; RNA Pull-Down; Actinomycin D / DRB Stability Assay; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; Western Blot; Clinical Sample Validation |
| Clinical significance: |
might predict poor survival in early-stage HCC |
| Description: |
In HCC, circANKRD52 (hsa_circ_0026939) functions as an oncogenic circRNA that promotes proliferation and invasion. It predominantly localizes to the cytoplasm and sponges miR-497-5p, thereby relieving repression of BIRC5 and enhancing tumorigenesis in vitro and in a cell line-derived xenograft model. |
| Confidence score: |
0.8294 |