| Expression pattern: |
UP |
| Associated gene: |
MMP13 |
| Associated microRNA: |
miR-136 |
| Biological function: |
Promotes ESCC cell proliferation and tumor growth; promotes migration, invasion and EMT; circRNA_100876 depletion induces apoptosis and causes G2/M arrest. |
| Molecular mechanism: |
Likely ceRNA mechanism involving miR-136 and MMP13; promotes EMT with changes in E-cadherin, N-cadherin, vimentin and Snail after knockdown. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Survival Analysis; Transfection; CCK8; Colony Formation Assay; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
High circRNA_100876 expression is associated with tumor invasion depth, lymph node metastasis, vascular invasion, shorter recurrence-free survival and overall survival, suggesting a prognostic biomarker in ESCC. |
| Description: |
circRNA_100876 is up-regulated in ESCC tissues and ESCC cell lines and higher expression predicts worse RFS/OS. Functional assays show that circRNA_100876 promotes proliferation, migration, invasion and EMT, while its knockdown induces apoptosis and G2/M arrest and suppresses xenograft tumor growth. The paper suggests a ceRNA-like mechanism involving miR-136 and MMP13. |
| Confidence score: |
0.6221 |