circRNA basic information
circBase ID: -
Name: mmu_circ_HIPK3
Synonym: circular RNA HIPK3
Host Gene: HIPK3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0024644
MONDO name: myocardial ischemia
Disease details: myocardial ischemia
Disease DO ID:
 -
Disease MeSH ID:
 D017202
Disease NCIt ID:
 C50625
Disease ICD11 ID:
 1964269418
Disease OMIM ID:
 -
Species: Mouse
Species details: Mus musculus
Tissue specimen:

mouse hearts
Cell lines:

Neonatal mouse ventricular cardiomyocytes; HEK293T
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: ATG7
Associated microRNA: miR-20b-5p
Biological function: promotes H/R-induced cardiomyocyte autophagy and apoptosis; increases ROS accumulation and cell injury
Molecular mechanism: ceRNA sponge mechanism: circHIPK3 sponges miR-20b-5p to relieve repression on ATG7, increasing ATG7 and enhancing autophagy/apoptosis
Biological pathway or process:

autophagy (promotes); apoptosis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Transfection; Western Blot; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; TEM; In Vivo Animal Model
Clinical significance:

potential therapeutic target for myocardial I/R injury
Description:

circHIPK3 is up-regulated in mouse myocardial I/R and cardiomyocyte H/R injury, and it promotes excessive autophagy, ROS accumulation, and apoptosis. Mechanistically, circHIPK3 sponges miR-20b-5p to increase ATG7 expression, forming a circHIPK3/miR-20b-5p/ATG7 axis that aggravates H/R-induced injury.
Confidence score:

0.5699
Other information
Title:

CircHIPK3 regulates the autophagy and apoptosis of hypoxia/reoxygenation-stimulated cardiomyocytes via the miR-20b-5p/ATG7 axis.

Journal: Cell death discovery
Published: 2021
PubMed ID: 33824287
Study type:

biological research
Data availability: The data used to support the findings of this study are included within the article.
Code availability: -