| Expression pattern: |
UP |
| Associated gene: |
CDCA3, EIF4A3 |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
promotes proliferation; promotes migration; promotes invasion; promotes tumor growth in vivo |
| Molecular mechanism: |
Acts as a competing endogenous RNA (miRNA sponge) for miR-145-5p to upregulate CDCA3; EIF4A3 promotes hsa_circ_101555 biogenesis via binding to flanking sequences of CSNK1G1 pre-mRNA. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Bioinformatics Analysis; Microarray; RT-qPCR; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis |
| Clinical significance: |
High hsa_circ_101555 expression in HCC was associated with overall survival; serum hsa_circ_101555 was significantly higher in HCC patients compared to healthy controls; higher in III-IV stage HCC compared to I-II stage patients. |
| Description: |
hsa_circ_101555 is up-regulated in HCC tissues/serum and HCC cell lines and mainly localizes to the cytoplasm. It promotes HCC proliferation, migration, invasion and xenograft growth by acting as a ceRNA that sponges miR-145-5p, thereby relieving repression of CDCA3; EIF4A3 facilitates its biogenesis via CSNK1G1 pre-mRNA binding. |
| Confidence score: |
0.8868 |