| Expression pattern: |
UP |
| Associated gene: |
ERalpha |
| Associated microRNA: |
miR-200a-3p |
| Biological function: |
Enhances tamoxifen resistance, promotes cell viability, migration/invasion (metastasis-related behavior), and modulates EMT markers and ERalpha level in breast cancer cells via exosomal transfer. |
| Molecular mechanism: |
Exosome-mediated transfer of circ_UBE2D2 directly binds (sponges) miR-200a-3p, thereby enhancing tamoxifen resistance and affecting ERalpha and EMT-related proteins. |
| Biological pathway or process: |
chemoresistance (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Transfection; CCK8; Transwell Assay; Western Blot; RNA Immunoprecipitation; Luciferase Reporter Assay; In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
Proposed as a biomarker and therapeutic target for tamoxifen-resistant (drug-resistant) ER-positive breast cancer. |
| Description: |
circ_UBE2D2 is up-regulated in tamoxifen-resistant breast cancer tissues/cells and is enriched in exosomes from resistant cells. Exosome-transferred circ_UBE2D2 binds miR-200a-3p and thereby promotes tamoxifen resistance, increasing cell viability and migration/invasion and modulating EMT markers and ERalpha in vitro and in xenograft tumors. |
| Confidence score: |
0.7581 |