| Expression pattern: |
UP |
| Associated gene: |
ZEB1 |
| Associated microRNA: |
miR-1236-3p |
| Biological function: |
promotes LUAD cell metastasis and induces EMT |
| Molecular mechanism: |
acts as a miRNA sponge for miR-1236-3p to relieve repression of ZEB1, thereby promoting EMT and metastasis |
| Biological pathway or process: |
EMT (promotes); metastasis (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Transfection; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; FISH / smFISH; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis; Back-Splice Junction PCR / divergent primers PCR |
| Clinical significance: |
may have important significance in the diagnosis of LUAD and may become biomarkers of LUAD in the future |
| Description: |
circ-HMGA2 (hsa_circ_0027446) is up-regulated in LUAD tissues and cells and promotes migration/invasion and lung metastasis by inducing EMT. It mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-1236-3p to increase ZEB1 expression, thereby activating an EMT program. |
| Confidence score: |
0.8455 |